Sfoglia per Autore
An Organelle Sizer Based on Local Image Correlation Spectroscopy Detects Changes in Subcellular Morphology
2016-01-01 Scipioni, Lorenzo; DI BONA, Melody; D'Amora, Marco; Gratton, E.; Diaspro, ALBERTO GIOVANNI; Lanzanò, L.
Application of the Split-FLCS Method to the detection of nanoscale diffusion in 3d in live cells
2016-01-01 Lanzanò, L.; Scipioni, Lorenzo; DI BONA, Melody; Bianchini, Paolo; Bizzarri, Ranieri; Cardarelli, F.; Vicidomini, Giuseppe; Diaspro, ALBERTO GIOVANNI
Role of the Pico- Nano-Second Temporal Dimension in STED Microscopy
2016-01-01 Lanzanò, L.; Scipioni, Lorenzo; Castello, Marco; Bianchini, Paolo; Vicidomini, Giuseppe; Diaspro, ALBERTO GIOVANNI
Phasor Analysis of Local ICS Detects Heterogeneity in Size and Number of Intracellular Vesicles
2016-01-01 Scipioni, Lorenzo; Gratton, E.; Diaspro, ALBERTO GIOVANNI; Lanzanò, L.
Measurement of nanoscale three-dimensional diffusion in the interior of living cells by STED-FCS
2017-01-01 Lanzanò, Luca; Scipioni, Lorenzo; Di Bona, Melody; Bianchini, Paolo; Bizzarri, Ranieri; Cardarelli, Francesco; Diaspro, Alberto; Vicidomini, Giuseppe
Three-dimensional multiple-particle tracking with nanometric precision over tunable axial ranges
2017-01-01 Sancataldo, Giuseppe; Scipioni, Lorenzo; Ravasenga, Tiziana; Lanzanò, Luca; Diaspro, Alberto; Barberis, Andrea; Duocastella, Martí
STED microscopy: Exploring fluorescence lifetime gradients for super-resolution at reduced illumination intensities
2018-01-01 Lanzano, L.; Vicidomini, G.; Scipioni, L.; Castello, M.; Diaspro, A.
Comprehensive correlation analysis for super-resolution dynamic fingerprinting of cellular compartments using the Zeiss Airyscan detector
2018-01-01 Scipioni, L.; Lanzano, L.; Diaspro, A.; Gratton, E.
Imaging of nuclear structures within intact eukaryotic nuclei is imperative to understand the effect of chromatin folding on genome function. Recent developments of super-resolution fluorescence microscopy techniques combine high specificity, sensitivity, and less-invasive sample preparation procedures with the sub-diffraction spatial resolution required to image chromatin at the nanoscale. Here, we present a method to enhance the spatial resolution of a stimulated-emission depletion (STED) microscope based only on the modulation of the STED intensity during the acquisition of a STED image. This modulation induces spatially encoded variations of the fluorescence emission that can be visualized in the phasor plot and used to improve and quantify the effective spatial resolution of the STED image. We show that the method can be used to remove direct excitation by the STED beam and perform dual color imaging. We apply this method to the visualization of transcription and replication foci within intact nuclei of eukaryotic cells.
2018-01-01 Sarmento, Mj.; Oneto, M.; Pelicci, S.; Pesce, L.; Scipioni, L.; Faretta, M.; Furia, L.; Dellino, G.; Pelicci, Pg.; Bianchini, P.; Diaspro, A.; Lanzano, L .
Local raster image correlation spectroscopy generates high-resolution intracellular diffusion maps
2018-01-01 Scipioni, Lorenzo; Di Bona, Melody; Vicidomini, Giuseppe; Diaspro, Alberto; Lanzanò, Luca
Local image correlation methods for the characterization of subcellular structure and dynamics by confocal and super-resolution microscopy
2018-02-12 Scipioni, Lorenzo
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