Analisi in vitro della citotossicità di materiali compositi per protesi “metal-free”.

The more and more frequent aesthetical requests from the patients have brought the actual dentistry to use, especially in prosthodontics, materials highly technological and reliable, from a physical-mechanic point of view, produced by the industry to such purpose and above all to an always smaller use of metallic substructures. But are such materials, both them composites or ceramics, also biocompatibles and biointegrables with the dental and periodontal structures ? Purpose of this study has been to investigate in vitro the degree of cytotoxicity of six composite materials used in metal-free FPD and precisely: Gradia, Estenia, Targis, Diamond Crown, Dei Clever Reply and Sculpture. The materials have been tested following the ISO 10993 guidelines, performing the in vitro cytotoxicity test with the use of cells of mouse, fibroblasto L929 (isolated by muscolar tissue) and cultivated in a fit ground of growth. The originality of this investigation is in the fact that the six composite materials, taken in examination, have been tested under the same conditions; the cytotoxicity test has been performed on these materials in contemporary way, in the same period, under the same conditions of temperature and humidity, by the same operator. All tested materials have been prepared and used according to the “clinical use” protocol: they are prepared directly from manufacturers and the finished up and polished with standard sequence from an only dental laboratory. All the tested composite materials have been shown no cytotoxicity.