The levels of adenine (ATP, ADP, AMP) and pyridine (NAD, NADH) nucleotides in human platelets were measured simply and reproducibly. A rapid alk. extn. allows complete recovery of the compds. concerned. The metabolic ATP and ADP in the cytosolic fraction, the amt. released upon thrombin stimulation, and the ADP bound to F-actin also were evaluated. Anal. was performed by reversed-phase, isocratic HPLC on a 5-μm Lichrosorb RP-18 column with UV detection at 254 nm.
Alkaline extraction and reverse-phase high-performance liquid chromatography of adenine and pyridine nucleotides in human platelets.
MAZZEI, MAURO;BALBI, ALESSANDRO
1987-01-01
Abstract
The levels of adenine (ATP, ADP, AMP) and pyridine (NAD, NADH) nucleotides in human platelets were measured simply and reproducibly. A rapid alk. extn. allows complete recovery of the compds. concerned. The metabolic ATP and ADP in the cytosolic fraction, the amt. released upon thrombin stimulation, and the ADP bound to F-actin also were evaluated. Anal. was performed by reversed-phase, isocratic HPLC on a 5-μm Lichrosorb RP-18 column with UV detection at 254 nm.
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