Campanula sabatia De Not.: primi risultati di propagazione in vitro per una specie protetta

Savona's bell (Campanula sabalia De Not.) is a paleomediterranean species belonging to the subsect. Hererophyllae of the genus Campanula. Nowaday the species is decreasing in presence in the sea cost area of the Region Liguria and owing to the heavy antropic pressure il was inclueleel in the list of endangered species. In order to perform a conservation strategy of this species the study of the possibility of in vitro multiplication was carried out. After the shoot sterilization a first medium for the adaptation to in vitro conditions was used; this medium was composed by the basal Murashige and Skoog medium (1962) plus BA 0.3 mg/L, ascorbic acid (100 mg/L) and citric acid (10 mg/L). A shoot multiplication experiment was performed with increasing concentrations of BA in order to obtain an interesting multiplication rate and a good quality of the explants. A 59.3% of sterilisation was obtained; the insertion of benzyladenine permitted to increase the multiplication rate up to a 4.2 shoots per explant in 20 days of culture. The presence of a green and hard callus at the base of the explants was observed; from this callus it is possible to induce shoot organogenesis. Preliminary rooting trials were performed and 88% of rooting percentage was obtained. The plantlets were successfully transferred to the greenhouse. These encouraging resulls could give rise to the possibility of reintroducing successfully the material in the originaI SIC.