To identify the most suitable operating conditions for xylose reductase (XR) activity of Debaryomyces hansenii UFV-170, a 2^2 central composite design face-centered (CCF) was used in this work, varying pH and temperature in the ranges 4–8 and 25–55°C, respectively. The results collected for two response variables, specifically the volumetric and the specific activities, were worked out by Response Surface Methodology. The XR stability was also investigated after storage at different temperatures.
Optimal activity and thermostability of xylose reductase from Debaryomyces hansenii UFV-170
CONVERTI, ATTILIO;
2009-01-01
Abstract
To identify the most suitable operating conditions for xylose reductase (XR) activity of Debaryomyces hansenii UFV-170, a 2^2 central composite design face-centered (CCF) was used in this work, varying pH and temperature in the ranges 4–8 and 25–55°C, respectively. The results collected for two response variables, specifically the volumetric and the specific activities, were worked out by Response Surface Methodology. The XR stability was also investigated after storage at different temperatures.
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