A general and simple method for 3-D image restoration in optical sectioning microscopy is presented. A set of images taken with an optical system with a known 3-D point spread function (psf) are considered. The restoration task is described, pointing out theoretical constraints on recovering the actual spatial distribution of the original object. A spatial-frequency analysis is performed, using no a priori information about the specimens udner investigation. The feasibility and limitations of 3-D reconstructions are analyzed. Moreover, a procedure is proposed that allows one to recover object projections within an angle range strictly dependent on the psf of the system used. 3-D objects have been simulated in order to produce a set of images similar to those provided by an optical system. Restoration has then been accomplished, and results demonstrate that side-views of an object can be obtained to a satisfactory degree of accuracy. Finally, a discussion concerning the presence of transparent and opaque objects in the field of view is also presented.

3-D RECONSTRUCTION IN OPTICAL MICROSCOPY BY A FREQUENCY-DOMAIN APPROACH

DIASPRO, ALBERTO GIOVANNI;BIANCO, BRUNO
1993-01-01

Abstract

A general and simple method for 3-D image restoration in optical sectioning microscopy is presented. A set of images taken with an optical system with a known 3-D point spread function (psf) are considered. The restoration task is described, pointing out theoretical constraints on recovering the actual spatial distribution of the original object. A spatial-frequency analysis is performed, using no a priori information about the specimens udner investigation. The feasibility and limitations of 3-D reconstructions are analyzed. Moreover, a procedure is proposed that allows one to recover object projections within an angle range strictly dependent on the psf of the system used. 3-D objects have been simulated in order to produce a set of images similar to those provided by an optical system. Restoration has then been accomplished, and results demonstrate that side-views of an object can be obtained to a satisfactory degree of accuracy. Finally, a discussion concerning the presence of transparent and opaque objects in the field of view is also presented.
File in questo prodotto:
Non ci sono file associati a questo prodotto.

I documenti in IRIS sono protetti da copyright e tutti i diritti sono riservati, salvo diversa indicazione.

Utilizza questo identificativo per citare o creare un link a questo documento: https://hdl.handle.net/11567/194069
Citazioni
  • ???jsp.display-item.citation.pmc??? ND
  • Scopus 16
  • ???jsp.display-item.citation.isi??? 10
social impact